Bioluminescence in the organs of mice pretreated with rabbit sera before infection with IHD-J-Luc. Mice were inoculated s. c. with anti-MV (purple), anti-EV (red) cocktails, or with QVPA (blue), NRS (green), or PBS (black) on day -1 and were infected with IHD-J-Luc VACV on day 0. Animals were subjected to whole-body imaging daily for 10 days. Bioluminescence in the nasal cavity (A, E), lungs (B, F), liver C, G), and spleen (D, H) was recorded and used to calculate mean total fluxes ± SD (A-D) and t-statistic (E-H). Axis "y" in panels E-H shows the value of t; t ≥ 2.16 is significant at α = 0.05 (two-tailed) for groups of 6 mice; t = 2.16 is depicted with broken horizontal line (E-H).