Figure 1From: Polyclonal antibody cocktails generated using DNA vaccine technology protect in murine models of orthopoxvirus diseaseSera from vaccinated NHP can protect mice from lethal VACV challenge. Groups of eight mice were injected subcutaneously with heat-inactivated sera collected from NHP previously vaccinated [34] with Dryvax (NHP ID# CH63 ref. x), 4pox DNA vaccine (NHP ID# CH93), L1R DNA vaccine (NHP ID# CH63), or an irrelevant DNA vaccine (NHP ID# CH02). As positive controls for protection, groups of eight mice were injected with 100 μg of purified anti-L1 murine monoclonal antibodies MAb-7D11 or MAb-10F5. As negative controls, eight mice received 100 μg of an irrelevant murine monoclonal antibody (MAb-3d7). One day after injection with NHP sera or murine MAbs, the mice were challenged with 2 × 106 PFUPFU of VACV strain IHD-J intransally. Percent survival (A) and percent group weight loss (B) were plotted. In this experiment, individual mouse weights were not recorded.Back to article page