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Figure 1 | Virology Journal

Figure 1

From: Polyclonal antibody cocktails generated using DNA vaccine technology protect in murine models of orthopoxvirus disease

Figure 1

Sera from vaccinated NHP can protect mice from lethal VACV challenge. Groups of eight mice were injected subcutaneously with heat-inactivated sera collected from NHP previously vaccinated [34] with Dryvax (NHP ID# CH63 ref. x), 4pox DNA vaccine (NHP ID# CH93), L1R DNA vaccine (NHP ID# CH63), or an irrelevant DNA vaccine (NHP ID# CH02). As positive controls for protection, groups of eight mice were injected with 100 μg of purified anti-L1 murine monoclonal antibodies MAb-7D11 or MAb-10F5. As negative controls, eight mice received 100 μg of an irrelevant murine monoclonal antibody (MAb-3d7). One day after injection with NHP sera or murine MAbs, the mice were challenged with 2 × 106 PFUPFU of VACV strain IHD-J intransally. Percent survival (A) and percent group weight loss (B) were plotted. In this experiment, individual mouse weights were not recorded.

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