Figure 9

Comparison of influenza virus infected and non-infected lung tissue and lectin staining. A: Bronchiole and lung parenchyma from swine influenza virus (SIV) H1N1 infected pig euthanized PID 4. SIV antigen positive epithelium (red) is demonstrated by immunohistochemistry using polyclonal goat anti-influenza A antibody and counterstaining with hematoxylin. In the same section it is possible to identify infected (arrow) and non-infected (arrowhead) bronchiolar epithelium. B: Parallel section to A demonstrating SNA lectin staining (dark blue) of the SA-α-2,6-terminal saccharide receptor. The lectin staining in the non-influenza infected part of the bronchiole (arrowhead) is considerable stronger compared to the infected part (arrow). C: Lung section from SIV H1N1 infected pig euthanized PID 4. Influenza positive cells (red) are demonstrated by immunohistochemistry using polyclonal goat anti-influenza A antibody and counterstaining with hematoxylin. In the same section it is possible to identify an infected (*) and non-infected lobules (#). D: Parallel section to C demonstrating SNA lectin staining. The lectin staining is reduced in the influenza infected area (*). E: Lung section from avian influenza (AIV) infected pig euthanized PID 4 stained for AIV antigens (red). In the same section it is possible to identify an infected (*) and non-infected lobulus (#). F: Parallel section to E demonstrating SNA lectin staining. A reduction of lectin staining is seen in the influenza infected lobulus (*) compared to the non-infected lobulus. (Original magnifications A, B, C, and D x10).