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Figure 2 | Virology Journal

Figure 2

From: Distribution of sialic acid receptors and influenza A virus of avian and swine origin in experimentally infected pigs

Figure 2

Lectin staining in nose, trachea, and lung from pigs. Examples of lectin staining demonstrating infAv receptors SNA lectin (SA-α-2,6-terminal saccharide) and MAAI and II lectin (SA-α-2,3-terminal saccharides), respectively, in nose, trachea, and lung of pigs. The lectin staining appear as a dark blue colour. A: Nasal epithelium demonstrating SNA lectin staining. A SNA lectin positive lining of epithelial cells (arrowhead) and SNA positive endothelial cells (arrow) are shown. B and C: Nasal epithelium stained with MAAI and MAAII lectin, respectively. It is not possible to recognise staining of the surface of the epithelium (arrowhead). D: Tracheal epithelium demonstrating SNA lectin staining. Positive staining is shown of the surface of the epithelium (arrowhead). E and F: Tracheal epithelium stained with MAAI and MAAII lectin, respectively. It is not possible to recognise staining of the surface of the epithelium (arrowhead). G: Lung section demonstrating SNA lectin staining. A SNA lectin positive lining of epithelial cells is seen in alveoli (a) and bronchioles (b). H: Lung section demonstrating MAAI lectin staining. Very few epithelial cells are positive in both bronchioles (b) and alveoli (a). I: Lung section demonstrating MAAII lectin staining, scattered positive reaction is shown in the alveolar epithelial cells (a) and few positive cells are seen in bronchioles (b). (Original magnifications A, B, C, D, E, and F: x40; G, H, and H: × 10).

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