Statistical analysis of ZANK amino acid sequence similarity to other ankyrins. Representative results of shuffling experiments for ZANK, vertebrate ankyrins, IκBα, p53BP2/ASPP2 ankyrins, and invertebrate ankyrins Cactus and Unc-22 are shown. Top: Randomizations of whole ankyrin repeats. Middle: ankyrin stem regions only. Bottom: ankyrin loop regions only. 10,000 randomly shuffled sequences of identical amino acid composition were generated in each cell. Shuffled sequences were aligned with indicated non-shuffled sequence and the number of scores greater than or equal to that of the non-shuffled input sequence is shown.. Significant alignments (p < 0.05) are shown highlighted in yellow while highly significant alignments (p < 0.001) are shown highlighted in red.