Figure 3

ZANK region amino acids correspond precisely with exon 3 of BZLF-1 gene encoding ZEBRA. DNA encoding the ZANK region and mRNA splice sites are shown. Using the same color scheme as in Figure 1, amino acids corresponding to the ZANK stem are shown in dark blue and those corresponding to the loop region are shown in light blue. DNA sequence is derived from the B-958 strain of EBV. A single amino acid polymorphism at aa 205 in this region has been identified resulting in a Ser substitution in certain strains of EBV such as Akata. Underlined sequences indicate regions of the putative ZANK loop present in the crystal structure. The splice points of ZEBRA exon 3 encoding ZANK is shown as "^" starting at the ZEBRA amino acid sequence "Val-Ala-Ala-Ala..." continuing to the end of the protein. The structured region of ZANK loop (Figure 1, 2) corresponds to ZANK sequences 227-230 Val-Asp-Ser-Ile. Residues His239 and Phe245 in the terminus of ZANK correspond to functionally conserved residues in the loops of other ankyrin proteins as discussed in more detail in the text.