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Table 3 Replication of the H10N8 virus from P0, P2, P4, P6 in mice§

From: Characterization of an H10N8 influenza virus isolated from Dongting lake wetland

Virus Strain

Days of post infection

Virus titre [log10(EID50)] in:

MLD50a(log10EID50)

  

brain

lung

spleen

kidney

 

P0

3

-

3.7 ± 0.9

-

-

>6.5

 

5

-

4.7 ± 1.5

-

-

 

P2

3

1.4 ± 0.8

6.7 ± 0.4

+

1.6 ± 0.4

4.7

 

5

+

6.3 ± 0.5

+

+

 

P4

3

2.0 ± 0.3

6.8 ± 0.5

3.4 ± 0.5

2.4 ± 0.6

3.6

 

5

ND

ND

ND

ND

 

P6

3

2.4 ± 0.7

6.6 ± 0.4

3.6 ± 0.3

3.7 ± 0.3

3.2

 

5

ND

ND

ND

ND

 
  1. § Six-week-old BALB/c mice were infected intranasal with 105.5EID50 of the viruses from different passage (P0,P2,P4,P6). Organs were collected on days 3 and 5 after infection, and clarified homogenates were titrated for virus infectivity in 10-day-old SPF embryonated chicken eggs.
  2. a The MLD50 dose was determined by inoculating groups of five 6-week-old female mice intranasally with 10-fold serial dilutions of each virus according to Reed and Muench method.
  3. - , Virus was not detected in the samples.
  4. +, Virus was simply detected in undiluted samples.
  5. ND, not done.