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Table 3 Replication of the H10N8 virus from P0, P2, P4, P6 in mice§

From: Characterization of an H10N8 influenza virus isolated from Dongting lake wetland

Virus Strain Days of post infection Virus titre [log10(EID50)] in: MLD50a(log10EID50)
   brain lung spleen kidney  
P0 3 - 3.7 ± 0.9 - - >6.5
  5 - 4.7 ± 1.5 - -  
P2 3 1.4 ± 0.8 6.7 ± 0.4 + 1.6 ± 0.4 4.7
  5 + 6.3 ± 0.5 + +  
P4 3 2.0 ± 0.3 6.8 ± 0.5 3.4 ± 0.5 2.4 ± 0.6 3.6
  5 ND ND ND ND  
P6 3 2.4 ± 0.7 6.6 ± 0.4 3.6 ± 0.3 3.7 ± 0.3 3.2
  5 ND ND ND ND  
  1. § Six-week-old BALB/c mice were infected intranasal with 105.5EID50 of the viruses from different passage (P0,P2,P4,P6). Organs were collected on days 3 and 5 after infection, and clarified homogenates were titrated for virus infectivity in 10-day-old SPF embryonated chicken eggs.
  2. a The MLD50 dose was determined by inoculating groups of five 6-week-old female mice intranasally with 10-fold serial dilutions of each virus according to Reed and Muench method.
  3. - , Virus was not detected in the samples.
  4. +, Virus was simply detected in undiluted samples.
  5. ND, not done.