Characterization of an H10N8 influenza virus isolated from Dongting lake wetland

Table 2 Pathotyping and replication of the H10N8 virus in chickens^§

Infection route	Days of post infection	Virus isolated from swabs				No.of Survivors	No.of Seroconverted Chickens^b	HI titers (Log₂)
		Oropharyngeal		Cloacal
		No.of Chickens shedding virus	Titer^a (log₁₀ EID₅₀/ml)	No.of Chickens shedding virus	Titer^a (log₁₀ EID₅₀/ml)
Intravenous (8)	3	3	1.7 ± 0.3	4	3.1 ± 0.7	8	8	6.3 ± 0.5
	5	7	3.8 ± 0.6	4	3.0 ± 0.7
	7	5	1.7 ± 0.5	3	1.6 ± 0.5
Intranasal(8)	3	5	2.3 ± 0.8	4	2.0 ± 0.3	8	8	5.6 ± 1.2
	5	8	3.4 ± 1.1	7	3.3 ± 0.1
	7	6	2.5 ± 0.9	5	1.3 ± 0.4

^§One group of 8 six-week-old specific-pathogen-free white leghorn chickens were inoculated with 0.2 ml of 1:10diluted stock virus (10^6.3 EID₅₀) intravenously and another group were inoculated with 10^6.0 EID₅₀ of the virus in a 0.1 ml volume intranasally, and observed for 2 weeks after infection.
^a The mean titer in EID₅₀/ml of swab media of the positive chickens.
^b Sera were harvested 3 weeks after infection, and seroconversion was confirmed by HI test.

ISSN: 1743-422X