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Figure 3 | Virology Journal

Figure 3

From: Characterization of the latent membrane protein 1 signaling complex of Epstein-Barr virus in the membrane of mammalian cells with bimolecular fluorescence complementation

Figure 3

BiFC Specificity with Decreased BiFC Construct Expression. LMP1-NYFP and LMP1-NYFP mutants were transfected with CYFP-TRAF2 and CYFP-TRAF3 (Panel A and B, respectively) as in Figure 2 except that ten-fold less BiFC plasmids were used. Cells were analyzed for BiFC by flow cytometry as in Figure 2. Representative YFP histograms of 1 × 104 mCherry positive cells are depicted. The YFP positive cells were gated as indicated and expanded in the lower panels. Histograms for LMP1-NYFP (blue), A5-Y384G-NYFP (orange), 1-231-NYFP (purple), 1-231-A5-NYFP (light green), 1-187-NYFP (dark green), and mCherry alone (no BiFC plasmids, red) are displayed. Percent YFP positive cells in the mCherry positive population is displayed in panel C.

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Virology Journal

ISSN: 1743-422X