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Table 4 Primers used in RT-PCR to amplify viral sequences obtained from Narcissus flowers

From: Colour break in reverse bicolour daffodils is associated with the presence of Narcissus mosaic virus

Primer name

Primer sequence

Potex1RC1 (For)

Potex51 (Rev)

5'-TCAGTRTTDGCRTCRAARGT-3'

5'-CAYCARCARGCMAARGAYGA-3'

Sprimer2 (For)

KS3(Rev)

5'-GGNAAYAAYAGYGGNCARCC-3'

5'-CGGTACCGATAAGCTTGA-3'

pCar-12(For)

KS3(Rev)

5'-ATGCCNCTNANNCCNCC-3'

5'-CGGTACCGATAAGCTTGA-3'

KSDT

5'-CGGTACCGATAAGCTTGATTTTTTTTTTTTTTTTTTV-3'

NMV57594 (For)

NMV5830(Rev)

5'-TGCGCTTCTATCTATTGTCTGAGG-3'

5'-GTGGGGAAGGGAATGAATGTTATC-3'

NLSYV13744 (For)

NLSYV1466(Rev)

5'-TACGACAGAAGAGAACACGGAGAG-3'

5'-ACACAATACTACACGCCCCTTACG-3'

  1. 1Degenerate primers of van der Vlugt & Berendsen [11], and 2Chen et al. [12]; 3generic primer that anneals with first strand cDNA synthesis primer, KSDT. N = (A, G, C or T), D = (G+A+T), Y = (T or C), R = (A or G). NMV = Narcissus mosaic virus, NLSYV = Narcissus late season yellows virus. 4 Primer regions in NMV (accession AY225449) and NLSYV (accession AJ493579). Numbers in subscript identify region in viral genome that primers bind. NMV primers are also perfect match for NMV accession D13747.