Colour break in reverse bicolour daffodils is associated with the presence of Narcissus mosaic virus

Table 1 Lesion formation occurrence in indicator plants following inoculation with extracts from colour-broken and non-colour-broken Narcissus flowers

Cultivar name	Location	Colour broken	Host reaction	Potyvirus DIBA-ELISA results
Twilight Zone	Ashhurst	Yes	LL on Gg turning systemic	-
		No	NS	(+)
Twilight Zone	Ashhurst	Yes	LL on Gg turning systemic LL on Nt	(+)
		No	NS	-
Twelve Gauge	Foxton	Yes	LL on Gg turning systemic LL Ca	-
		No	NS	-
Daydream	Nelson	Yes	LL on Gg turning systemic	-
		No	NS	-
Startracker	Ashhurst	Yes	LL on Gg turning systemic	-
		No	NS	-
Honeybird	Foxton	Yes	LL on Gg turning systemic	-
		No	NS	(+)
82/90 (Rich Reward × [Daydream × Empress of Ireland])	Nelson	Yes	LL on Gg turning systemic	-
		No	NS	-
20/87 (Royalist × [1W P Sdg] × Fintona) × Kabonova	Nelson	Yes	LL on Gg turning systemic	-
Daydream Mitsch	Nelson	Yes	LL on Gg turning systemic	(+)
Lighthouse Reef	Ashhurst	Yes	LL on Gg turning systemic	-
Trumpet Warrior	Nelson	No	NS	-
Gold Convention (non reverse bicolor)	Ashhurst	No	NS	+
Control PVY			-	+
Control sap			-	-
LL C am. Twelve Gauge				-
LL G g Daydream				-

Indicator plants Chenopodium amaranticolor (Ca), Gomphrena globosa (Gg), Phaseolus vulgaris (Pv) and Nicotiana tabacum (Nt) were inoculated with tepal tissue extracts and the host reaction determined. NS = no symptoms; LL = local lesion. Presence of potyviruses in the tepal extracts was determined by DIBA-ELISA. All daffodil tepals were tested. '+' = positive, '(+)' = weak positive reaction, '-' = negative

ISSN: 1743-422X