Figure 5
From: Colour break in reverse bicolour daffodils is associated with the presence of Narcissus mosaic virus
RT-PCR with NMV- and NLSYV-specific primers on RNA isolated from colour-broken and non-colour-broken Narcissus tepals. Thirty cycles of PCR were performed using primers listed in Table 1. Expected size in bp for the amplified products is indicated. Flowers were supplied from two different regions in New Zealand (Nelson, Lanes 4-7 and 12-13; Ashhurst, Lanes 2-3 and 8-11). Lanes 2, 4, 6, 8, 10, 12 show amplification from colour-broken flowers. Lanes 3, 5, 7, 9, 11, 13 from non-colour-broken flowers. Flower cultivars were Lighthouse Reef (lanes 2, 3); Daydream (4, 5); 20/87 seedling (6, 7); Twilight Zone (8, 9); Flying Cloud (10, 11) and Twilight Zone (12, 13). Molecular size markers on left are from 1 Kb Plus DNA Ladder (Invitrogen).