Figure 4
From: Colour break in reverse bicolour daffodils is associated with the presence of Narcissus mosaic virus
Amplification of putative viral RNA from tepal extracts of colour-broken and non-colour-broken Narcissus flowers. Thirty cycles of PCR were performed on first strand cDNA using degenerate primers for (A) potexviruses, (B) potyviruses and (C) carlaviruses. The approximate size of the PCR product expected is indicated by a star and arrows indicate the sizes of the amplified products sent for sequencing. Flowers were supplied from two different regions in New Zealand (Nelson, Lanes 4-7 and 12-13; Ashhurst, Lanes 2-3 and 8-11). Lanes 2, 4, 6, 8, 10, 12 show amplification from colour-broken flowers. Lanes 3, 5, 7, 9, 11, 13 from non-colour-broken flowers. Flower cultivars were Lighthouse Reef (lanes 2, 3); Daydream (4, 5); 20/87 seedling (6, 7); Twilight Zone (8, 9); Flying Cloud (10, 11) and Twilight Zone (12, 13). Molecular size markers on left are from 1 Kb Plus DNA Ladder (Invitrogen, Carlsbad, CA).