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Table 1 Primers used for amplification of the genome of PRRSV HuN4-F112.

From: Generation of an infectious clone of HuN4-F112, an attenuated live vaccine strain of porcine reproductive and respiratory syndrome virus

Primera

Sequencesb (5'-3')

Position in HuN4

For fragment A

  

F16(sp6)

CCGCTCGAGTTAATTAAATTTAGGTGACACTATA GG ATGACGTATAGGTGTT

1-16

R2355

GTGATGAACCTCGTCACCTTGTGCAGGG

2355-2382

For fragment B

  

F2300

CTTTGGGCAAGGACTCGGT

2300-2319

R5914

GATCCTGTGTGAACGCCGAC

5914-5933

For fragment C

  

F5853

CTTCTGCTTCACCGCGTGT

5853-5871

R8825

AAGAAGATTGGCGGCAAAC

8825-8843

For fragment D

  

F8764

GCAGGTGCCTTGAAGCTGAT

8764-8783

R11910

CTCATGCTGATGGCATTAGC

11910-11929

For fragment E

  

F11851

AGGACTGGGAGGATTACAAT

11851-11870

R14670

CGGACGACAAAC GCGTGGTTAT

14670-14691

For fragment F

  

F14668

TGATAACCACGCG TTTGTCGTC

14668-14689

R15313

TATAGCGGCCGCATTTAAAT(T)32AATTACGG

15313-15320

  1. a Primer names are organized in groups. Prefixes: F, forward PCR primer; R, reverse PCR primer.
  2. b The SP6 RNA polymerase promoter sequence in primer F16 is shown in italics. Restriction sites introduced by PCR are underlined and specified in parentheses at the end of the sequence. Silent mutations within the viral sequence are shown in boldface. Fragments A through F correspond to the letters in Fig. 1.