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Table 1 Testing of T0 and T1 transgenic plants for TMV or CMV resistance.

From: Virus-derived transgenes expressing hairpin RNA give immunity to Tobacco mosaic virus and Cucumber mosaic virus

T0 line number

T0 reaction to TMVa

T0 reaction to CMV

T0 copy number of transgene (Southern)

T1 segregation immune:susceptible

MP16

Immune

 

1

36:14*

MP31

Immune

 

>3

31:19

MP39

Immune

 

2

39:11

MP53

Immune

 

2

40:10

MP36

Susceptible

 

1

0:50

Rep15

 

Immune

1

38:12*

Rep17

 

Immune

2

30:20

Rep25

 

Resistant

>5

24:26

Rep53

 

Resistant

>3

29:21

Rep727

 

Susceptible

3 ~ 4

0:50

  1. aImmune indicated no detectable symptom and no virus particles were detected. Resistant indicated mild symptom and virus was detected. Susceptible indicated clear mosaic symptoms in the entire leaves.
  2. * The segregation for TMV or CMV resistance vs. susceptibility conforms to a 3: 1 ratio for a single dominant locus (χ2 test, P > 0.05).
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Virology Journal

ISSN: 1743-422X