Sequencing of bovine herpesvirus 4 v.test strain reveals important genome features

Palmeira, Leonor; Machiels, Bénédicte; Lété, Céline; Vanderplasschen, Alain; Gillet, Laurent

doi:10.1186/1743-422X-8-406

Virology Journal

Table 3 Potential BoHV-4 V.test ORFs and homologues to HHV-8 and HHV-1

From: Sequencing of bovine herpesvirus 4 v.test strain reveals important genome features

BoHV-4 ORF	Strand	Start^a	Stop^a	HHV-8 homologue^b	HHV-1 homologue^c	Annotation and comments
Bo1	-	272	460	--	--	Early in-frame STOP codon
*ORF 3*	+	*441*	*4307*	--	--	*BORFA1; v-FGAM-synthase*
Bo2	+	4435	4638	--	--
Bo3	+	5072	5299	--	--
*ORF 6*	+	*5703*	*9107*	*ORF 6*	*UL 29*	*single-stranded DNA-binding protein MDBP*
ORF 7	+	9112	11190	ORF 7	UL 28	transport protein
ORF 8	+	11180	13804	ORF 8	UL 27	glycoprotein B
*ORF 9*	+	*13944*	*16961*	*ORF 9*	*UL 30*	*DNA polymerase*
*ORF 10*	+	*17057*	*18337*	*ORF 10*	--	*BORFB1*
*Bo4*	-	*18604*	*19101*	--	--	*short ORF of immediate early transcript 1*
Bo5	-	19170	20355	K5	--	long ORF of immediate early transcript 1
Bo6	+	20297	20590	--	--	Early in-frame STOP codon
Bo7	-	20670	20994	--	--	Disrupted frame
Bo8	+	21318	21521	--	--	overlapping with late 1.7 kb RNA
*ORF 16*	+	*22967*	*23647*	*ORF 16*	--	*BORFB2; v-Bcl-2 protein*
ORF 17	-	23710	25260	ORF 17	UL 26	capsid protein
*ORF 18*	+	*25259*	*26077*	*ORF 18*	--
ORF 19	-	26029	27705	ORF 19	UL 25	tegument protein
ORF 20	-	27407	28219	ORF 20	UL 24
*ORF 21*	+	*28203*	*29540*	*ORF 21*	*UL 23*	*thymidine kinase*
ORF 22	+	29551	31674	ORF 22	UL 22	glycoprotein H
*ORF 23*	-	*31671*	*32873*	*ORF 23*	--
*ORF 24*	-	*32851*	*35109*	*ORF 24*	--
*ORF 25*	+	*35099*	*39220*	*ORF 25*	*UL 19*	*major capsid protein*
ORF 26	+	39256	40170	ORF 26	UL 18	capsid protein
ORF 27	+	40184	40829	ORF 27	--
Bo9	+	40831	41130	--	--
*ORF 29*	-	*41154*	*46328*	*ORF 29*	*UL 15*	*cleavage/packaging protein*
*ORF 30*	+	*42306*	*42548*	*ORF 30*	--
*ORF 31*	+	*42482*	*43123*	*ORF 31*	--
*ORF 32*	+	*43069*	*44439*	*ORF 32*	*UL 17*	*viral DNA cleavage/packaging protein*
*ORF 33*	+	*44432*	*45430*	*ORF 33*	*UL 16*
*ORF 34*	+	*46327*	*47313*	*ORF 34*	--
*ORF 35*	+	*47285*	*47758*	*ORF 35*	--
ORF 36	+	47787	48950	ORF 36	UL 13	kinase
ORF 37	+	48958	50427	ORF 37	UL 12	alkaline exonuclease
*ORF 38*	+	*50379*	*50585*	*ORF 38*	--
ORF 39	-	50652	51761	ORF 39	UL 10	glycoprotein M
*ORF 40*	+	*51877*	*53247*	*ORF 40*	*UL 8*	*helicase-primase complex component*
*ORF 41*	+	*53360*	*53881*	*ORF 41*	--	*helicase-primase complex component*
ORF 42	-	53873	54748	ORF 42	UL 7
ORF 43	-	54525	56375	ORF 43	UL 6	capsid protein
ORF 44	+	56323	58701	ORF 44	UL 5	helicase
ORF 45	-	58805	59530	ORF 45	--
ORF 46	-	59530	60291	ORF 46	UL 2	uracil-DNA-glycosidase
*ORF 47*	-	*60309*	*60731*	*ORF 47*	--	*glycoprotein L*
*ORF 48*	-	*60838*	*62382*	*ORF 48*	--
*ORF 50*	+	*62586*	*65179*	*ORF 50*	--	*immediate early transcript 2; R transactivator protein*
ORF 49	-	62600	63499	ORF 49	--
Bo10	+	65696	66595	--	UL 54	glycoprotein gp80
ORF 52	-	66621	67007	ORF 52	--
*ORF 53*	-	*67073*	*67345*	*ORF 53*	--
ORF 54	+	67414	68262	ORF 54	UL 50	dUTPase
*ORF 55*	-	*68321*	*68923*	*ORF 55*	--
ORF 56	+	68887	71418	ORF 56	UL 52	DNA replication protein
*ORF 57*	+	*71512*	*72870*	*ORF 57*	--	*possible post-transcriptional transactivator*
ORF 58	-	73294	74346	ORF 58	--
*ORF 59*	-	*74360*	*75535*	*ORF 59*	--	*DNA replication protein*
*ORF 60*	-	*75688*	*76605*	*ORF 60*	*UL 40*	*ribonucleotide reductase small subunit*
ORF 61	-	76639	79020	ORF 61	UL 39	ribonucleotide reductase large subunit
*ORF 62*	-	*79002*	*80021*	*ORF 62*	*UL 38*	*assembly/DNA maturation protein*
*ORF 63*	+	*79978*	*82797*	*ORF 63*	--	*tegument protein*
*ORF 64*	+	*82812*	*90488*	*ORF 64*	*UL 36*	*tegument protein*
*ORF 65*	-	*90504*	*90896*	*ORF 65*	--	*capsid protein*
*ORF 66*	-	*90893*	*92167*	*ORF 66*	--
*ORF 67*	-	*92107*	*92877*	*ORF 67*	*UL 34*	*tegument protein*
*ORF 67.5*	-	*92881*	*93144*	*ORF 67.5*	*UL 33*	*Disrupted (late) methionine*
ORF 68	+	93155	94504	ORF 68	UL 32	probable glycoprotein
ORF 69	+	94512	95405	ORF 69	UL 31
Bo11	-	96158	96703	--	--
Bo12	+	97442	97684	--	--	Early in-frame STOP codon
*ORF 71*	-	*98328*	*98876*	*K13/ORF 71*	--	*BORFE2; v-FLIP*
Bo13	+	98838	98983	--	--	Disrupted frame
ORF 73	-	99022	99783	ORF 73	--	BORFE3, LANA homologous
*ORF 75*	-	*100274*	*103606*	*ORF 75*	--	*Disrupted (late) methionine; tegument protein/v-FGAM-synthetase*
Bo14	-	104273	104785	--	--
Bo15	-	105724	106038	--	--
Bo16	+	106225	106494	--	--
*Bo17*	+	*106681*	*108003*	--	--	*viral beta-1,6-N-acetylglucosaminyltransferase*

^a Positions of the respective ORFs of BoHV-4 on the LUR sequence. These are given from the first nucleotide of the start codon ATG to the last nucleotide of the stop codon.
^b Correspondance to HHV-8 genes is according to Zimmermann (2001).
^c Correspondance to HHV-1 genes was based on the presence of conserved domain with a BoHV-4 gene. Genes containing evolutionary conserved domains are highlighted in bold italic. The genes conserved in Herpesviridae are highlighted in bold (from Fu, 2008).

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ISSN: 1743-422X

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