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Table 2 Potential BoHV-4 V.test ORFs presenting conserved functional domains

From: Sequencing of bovine herpesvirus 4 v.test strain reveals important genome features

BoHV-4 ORF

Domain accession number

Functional annotation

Species distributiona

ORF 3

COG0046, TIGR01735, TIGR01736, TIGR01739, PF02769

Phosphoribosylformylglycinamidine (FGAM) synthase; AIR synthase related protein

Eukaryota; Bacteria; Archaea; Viruses

ORF 6

PF00747

ssDNA binding protein

Herpesviridae

ORF 7

PF01366

Protein transporter activity

Herpesviridae

ORF 8

PF00606

Surface glycoprotein

Herpesviridae

ORF 9

COG0417, TIGR00592, PF00136, PF03104, SM00486

DNA polymerase type-B family

Eukaryota; Bacteria; Archaea; Viruses

ORF 10

PF04797

dUTPase

Herpesviridae

Bo4

COG5183, SM00744

Protein involved in mRNA turnover and stability; zinc-finger RING-variant domain

Eukaryota; Bacteria

ORF 16

SM00337

BCL (B-Cell lymphoma); contains BH1, BH2 regions

Eukaryota; Bacteria; Viruses

ORF 17

PF00716

Serine-type endopeptidase activity

Herpesviridae

ORF 18

PF03049

UL79 family

Herpesviridae

ORF 19

PF01499

Virus penetration and capsid assembly

Herpesviridae

ORF 20

PF01646

UL24 family

Herpesviridae

ORF 21

PF00693, PF01712, PF08465

ATP binding; thymidine kinase activity; phosphotransferase activity

Eukaryota; Bacteria; Viruses

ORF 22

PF02489

Virion associated envelope glycoprotein

Herpesviridae

ORF 23

PF04682

BTRF1 protein conserved region

Herpesviridae

ORF 24

PF03043

UL87 family

Herpesviridae

ORF 25

PF03122

Structural molecule activity

Herpesvirales

ORF 26

PF01802

Structural molecule activity

Herpesvirales

ORF 29

PF02499, PF02500

Probable role in DNA packaging

Herpesvirales

ORF 30

PF05338

Unknown function (DUF717)

Gammaherpesvirinae

ORF 31

TIGR01234, PF03048

UL92 family; L-ribulokinase

Herpesvirales; Embryophyta

ORF 32

PF04559

DNA cleavage and packaging

Herpesviridae

ORF 33

PF03044

Possible role in capsid maturation

Herpesviridae

ORF 34

PF03038

UL95 family

Herpesviridae

ORF 35

PF05852

Unknown function (DUF848)

Gammaherpesvirinae

ORF 36

COG0515, PF00069, SM00220

ATP binding; protein kinase activity; Serine/threonine protein kinase

Eukaryota; Bacteria; Archaea

ORF 37

PF01771, PF09588

Exonuclease activity; DNA binding

Eukaryota; Bacteria; Viruses

ORF 38

PF10813

Unknown function (DUF2733)

Herpesviridae

ORF 39

PF01528

Integral membrane protein

Herpesviridae

ORF 40

PF03324

Helicase-primase complex associated protein

Herpesviridae

ORF 41

PF05774

Helicase-primase complex components

Gammaherpesvirinae

ORF 42

PF01677

UL7-like protein

Herpesviridae

ORF 43

PF01763

Possible role in cleavage and packaging

Herpesviridae

ORF 44

COG0507, PF02689

Helicase; ATP-dependent exoDNAse (exonuclease V)

Eukaryota; Bacteria; Viruses

ORF 46

COG0692, TIGR00628, TIGR03443, PF03167

Uracil DNA glycosylase

Eukaryota; Bacteria; Archaea; Viruses

ORF 47

PF11108

Glycoprotein L

Herpesviridae

ORF 48

PF05734

Unknown function (DUF832)

Herpesviridae

ORF 50

PF03326, PF04793

Early-intermediate transcription factors

Gammaherpesvirinae

Bo10

PF05459, PF05812

Transcriptional regulator proteins

Herpesviridae

ORF 53

PF03554

Highly polymorphic glycoprotein

Herpesviridae

ORF 54

COG0756, TIGR00576, PF00692

dUTPase

Eukaryota; Bacteria; Archaea; Viruses

ORF 55

PF04533

U44 protein

Herpesviridae

ORF 56

PF03121

UL52/UL70 DNA primase

Eukaryota; dsDNA Viruses

ORF 57

PF04633

BMRF2 protein

Gammaherpesvirinae

ORF 59

PF04929

DNA replication accessory factor

Gammaherpesvirinae

ORF 60

COG0208, PF00268

Ribonucleotide reductase

Eukaryota; Bacteria; Archaea; Viruses

ORF 61

COG0209, TIGR02504, TIGR02506, TIGR02510, PF00317, PF02867

Ribonucleotide reductase

Eukaryota; Bacteria; Archaea; Viruses

ORF 62

PF03327

Capsid shell protein VP19C

Herpesviridae

ORF 63

PF04523

Tegument protein U30

Herpesviridae

ORF 64

PF04843

Tegument protein, N-terminal conserved region

Herpesviridae

ORF 65

PF06112

Capsid protein

Gammaherpesvirinae

ORF 66

PF03117

UL49 family

Herpesviridae

ORF 67

PF04541

Virion protein U34

Herpesviridae

ORF 67.5

PF03581

UL33-like protein

Herpesviridae

ORF 68

PF01673

Putative major envelope glycoprotein

Herpesviridae

ORF 69

PF02718

UL31-like protein

Herpesviridae

ORF 71

PF01335, SM00031

Death effector domain

Eukaryota; Viruses

ORF 75

COG0046, COG0047, TIGR01735, TIGR01736, TIGR01739, TIGR01857, PF02769

Phosphoribosylformylglycinamidine (FGAM) synthase; AIR synthase related protein

Eukaryota; Bacteria; Archaea; Viruses

Bo17

PF02485

Core-2/I-Branching enzyme

Eukaryota; Bacteria; Viruses

  1. Conserved functional domains were determined for each BoHV-4 V.test ORF using METHOD and the domains accession numbers, functional annotation and the species distribution were listed. When several domains were conserved, the annotations were either merged when possible or juxtaposed. Domains present in herpesviridae conserved gene families are highlighted in bold (from Fu, 2008). (PFxxx: Pfam Accession Number; SMxxx: SMART Accession Number; COGxxx: COG Accession Number; TIGRxxx: TIGRFam Accession Number).
  2. a Major taxonomic groups.