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Figure 3 | Virology Journal

Figure 3

From: Synthetic long oligonucleotides to generate artificial templates for use as positive controls in molecular assays: drug resistance mutations in influenza virus as an example

Figure 3

Schematic representation of generating large DNA molecule using synthetic oligonucleotide templates. (A) The formation of a dimer from two synthetic oligonucleotide templates. (B) In the presence of dNTP and DNA polymerase, each oligonucleotide will extend its 3' end using other oligo as a template and form double-stranded DNA molecules. (C) The theory can be further extended using multiple oligos containing complementary sequences at the 5'-phospahte ends. (D) Upon DNA polymerase reaching the 5' end an oligo where a second down stream oligo is annealed, the adjacent 3'-hydroxy ends and 5'-phosphate (P) can be sealed by DNA ligase via nick-sealing reactions. This process in theory can be used to generate a very large, double-stranded DNA fragment.

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Virology Journal

ISSN: 1743-422X