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Figure 2 | Virology Journal

Figure 2

From: Synthetic long oligonucleotides to generate artificial templates for use as positive controls in molecular assays: drug resistance mutations in influenza virus as an example

Figure 2

Ligase chain reaction testing standard containing resistance template at various levels. A: N1: 274Tyr (resistance)/His (wild type); B: H5N1: 274Tyr (resistance)/His (wild type). C: N2:119Val(resistance)/Glu(wild type), D: N2:292Lys(resistance)/Arg(wild type). After 10 cycle of ligation reaction, the generation of positive product can be detected even in the presence of low-level of resistance mutation (~1%).

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Virology Journal

ISSN: 1743-422X