Figure 2
From: Latent HIV in primary T lymphocytes is unresponsive to histone deacetylase inhibitors
Levels of HIV-p24 in culture supernatants post-stimulation of latent HIV. About 0.5 × 106 of long-term cultured HIV-infected CD4 T cells that include latently-infected cells were treated with various concentrations of prostratin (A), VPA (B) and TSA (C) for 72 h. Culture supernatants were harvested 24 h and 72 h post-treatment and assayed for HIV-p24 levels by using p24-ELISA kit (Advanced Bioscience Laboratories, Inc., USA). The relative levels of HIV-p24 in various samples were expressed in O.D. values. Only prostratin, at 500 nM or higher concentrations but not VPA or TSA, could increase the levels of p24 in culture supernatants.