Figure 6

ZsGreen-tagged Vpr localizes at the perinuclear rim within TF-1 bone marrow progenitor cells. (A) ZsGreen protein localized uniformly intracellularly (including nucleoli). (B and C) Both ZsGreen-Vpr and Vpr-ZsGreen display an intracellular localization pattern similar to that observed in HEK 293T cells. Vpr is found primarily at the nuclear envelope, although a low level of accumulation was also found in speckles both in the nucleus and in the cytoplasm. Similar to 293T cells, localized herniations were observed at the nuclear rim. The first, second, and third columns represent DAPI, FITC, and merged views, respectively. All figures denote a cross-sectional view of the cell and are representative of the entire cell population.