Skip to main content

Table 3 Primers and probes used for qPCR analyses

From: IPNV with high and low virulence: host immune responses and viral mutations during infection

Genes

Assay

Primer

Sequence (5'-3')

PCR efficiency

GenBank accession #

EF1aB

TaqMan

Forward

Reverse

Probe

TGCCCCTCCAGGATGTCTAC

CACGGCCCACAGGTACTG

AAATCGGCGGTATTGG

2.0

BG933897

IFN-c

TaqMan

Forward

Reverse

Probe

TGGGCAGTGTGGATACAAGTG

CTGCAATGTTCCCAAAGTACGTATT

CTGTCCTGATGAGATAAT

2.0

EU735545

EU735547-50

IFN-a1

TaqMan

Forward

Reverse

Probe

CCTTTCCCTGCTGGACCA

TGTCTGTAAAGGGATGTTGGGAAAA

CTTTGTGATATCTCCTCCCATC

1.94

AY2169594

AY2169595

IFN-γ

TaqMan

Forward

Reverse

Probe

AAGGGCTGTGATGTGTTTCTG

TGTACTGAGCGGCATTACTCC

TTGATGGGCTGGATGACTTTAGGA

2.0

AY795563

Mx1/2 e/e

TaqMan

Forward

Reverse

Probe

GATGCTGCACCTCAAGTCCTATTA

CGGATCACCATGGGAATCTGA

CAGGATATCCAGTCAACGTT

2.0

U66475

U66476

MyD88

TaqMan

Forward

Reverse

Probe

GACAAAGTTTGCCCTCAGTCTCT

CCGTCAGGAACCTCAGGATACT

CTGGTGCCCGGAGCAA

1.84

EF672332

TLR8

TaqMan

Forward

Reverse

Probe

ACCAAAACCACTAATGACATCATCTTCA

TGGTGATGCCATCAGGTATGTTT

CTCAGTCGACGCTCCTC

1.7

FJ467615

TLR9

TaqMan

Forward

Reverse

Probe

TCTATGGCTGGGATGTCTGGTA

CAGTTGTGAGTAGCCCTTGTGT

CAGCACCTGGAAGCAG

1.81

EF672331

TLR22

TaqMan

Forward

Reverse

Probe

ATTTATCCCGGAATCCATGTATCACG

CCACAGTAGGCGATGTCTAACA

CCTCAAGATAAGGAAGAACAT

1.98

AM233509

VP2 (IPNV)

TaqMan

Forward

Reverse

Probe

GCCAAGATGACCCAGTCCAT

TGACAGCTTGACCCTGGTGAT

CCGACCGAGAACAT

2.0

AJ877117

Beta 2 microglobulin

SybrGreen

Forward

Reverse

TCGTTGTACTTGTGCTCATTTACAGC

CAGGGTATTCTTATCTCCAAAGTTGC

1,6

BX076608

Galectin-3 binding protein

SybrGreen

Forward

Reverse

CCAGACCAACAGTGTTCACTTCAGC

ACGTGAAAGACATACCTGCCCTCAC

1,84

BX079375

Galectin-9

SybrGreen

Forward

Reverse

GTCCTGTCTATTGCCTTCTCCAACC

GGTTTCGTTGACCACTGTGTGGA

1,76

CU071943

MHC-IHC

SybrGreen

Forward

Reverse

CTGCATTGAGTGGCTGAAGA

GGTGATCTTGTCCGTCTTTC

1,72

CU070775

Pre-B cell ef

SybrGreen

Forward

Reverse

GACTTCAATTTCCTGCTGGCTA

CTGCTTGTAATGTGTGACCT

1.77

CA376673

RIG-I

SybrGreen

Forward

Reverse

GACGGTCAGCAGGGTGTACT

CCCGTGTCCTAACGAACAGT

1.97

DY714827

MDA5

SybrGreen

Forward

Reverse

CAGAGGTGGGGTTCAATGAT

AGCTCGCTCCACTTGTTGAT

1.92

FN396357

PKR

SybrGreen

Forward

Reverse

TTCCTGCATGGACTTGACTG

GTGAGGAACCGGTGTTCTGT

1.87

BT046111

SRK2

SybrGreen

Forward

Reverse

TAGACATGGCACCATGGACCCTC

GGGTTCTTCAGTGCAGACAGCCA

1.81

CA349577