Figure 9
Agarose gel electrophoresis of nested RT-PCR amplification of intracellular HCV RNA following incubation with decreasing concentrations of anti E2-epitopes caprine antibodies. Huh7.5 cells were infected Invitro using a pool of 10 HCV genotype 4 positive sera with viral loads ranges (9 × 105-1.5 × 106 copies/ml) in absence (lane 1) and presence (lanes 2-10) of serial dilutions (10 - 2.5 ug/ml) of caprine Abs. Cellular RNA was amplified using HCV 5'UTR specific nested primers as described in Materials and Methods. Agarose gel shows the status of Ab inhibition; anti p430 (A, lanes 2, 3, 4), anti p517 (B, lanes 5, 6, 7) and anti p412 (C, lanes 8, 9, 10). M is a molecular weight standard DNA marker (100 basepair; Jena Bioscience, Germany). Lane 11 shows the results of normal non infected Huh7.5 cells (negative control).