Western blott analysis to test the efficacy of anti p412 caprine antibody to recognize the corresponding intracellular viral antigens. Huh7.5 cells were infected with pooled HCV positive sera. Cell lysates were subjected to western blotting analysis using caprine anti p412 antibody. Two proteins of molecular weights 115kDa and 70kDa appeared only in HCV infected cells (lane 1) but not in uninfected cells (lane 2). M represents the Protein marker (Blue Ranger Prestained Marker Mix; Pierce, U.S.A).