Figure 3

Agarose gel electrophoresis of nested RT-PCR products to evaluate HCV infection into Huh7.5 cells from HCV positive sera. The HCV nested RT-PCR products at 174 bp band were visualized on 2% agarose gels stained with ethidium bromide. Lane 1: viral positive strand in the HCV genotype 4 patients' sera. Lane 2: viral positive strand in the patients' PBMCS. Lane 3: negative strand in the patients' PBMCS. Lane 4: nested RT-PCR product of the last wash of Huh7.5 cells to make sure there isn't ny residual virus from the infectious serum. Lane 5: viral positive strand in the cells 3 days post infection. Lane 6: viral negative strand in the cells 3 days post infection. Lane 7: viral positive strand in the supernatant 3 days post infection. Lane 8: viral negative strand in the cells' supernatant. M: molecular weight standard DNA marker (100 base pair; Jena Bioscience, Germany).