Two step partial purification of caprine IgG's from E2 synthetic peptide immunized caprines. Caprine sera before (lane 1) and after purification of p412, p430 and p517 (lanes 1, 2 and 3 respectively) immunized caprine sera were resolved on SDS PAGE followed by Coomassie blue staining. The caprine albumin appears as a dense band at ~ 64 kDa in unpurified serum and much less dense in purified sera. Lanes 1, 2 and 3 show distinct bands of heavy and light chain IgG at 53 and 26 kDa after purification. Lane M represents the Broad Range Protein Molecular Weight Marker (Promega; Madison, USA).