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Figure 6 | Virology Journal

Figure 6

From: Japanese Encephalitis Virus wild strain infection suppresses dendritic cells maturation and function, and causes the expansion of regulatory T cells

Figure 6

Effects of P3 infection on DCs-induced differentiation of regulatory T cells. 1 × 105 mock-, P3-, UV-P3- or LPS-treated bmDCs were incubated with 1 × 106 allogeneic naïve T cells for 5 days. T cells were purified and doubly labeled for CD4 and Foxp3, and assessed by FACS. The in vivo Treg in splenocytes were purified and examined by FACS from mice inoculated with 1 × 105 PFU P3 or identical UV-P3 i.p. for 5 days. Representative result was shown from three independent experiments (A). The percentage represented the ratio of CD4+ Foxp3+ cells in CD4+ T cells. P3-infected bmDCs elicited the Treg differentiation in vitro (B). After P3 infection or UV-P3 stimulation of mice i.p., Treg differentiation in vivo was analyzed immediately (C). Results were expressed as the means ± SD of triplicates. *, P < 0.05.

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Virology Journal

ISSN: 1743-422X