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Figure 4 | Virology Journal

Figure 4

From: Japanese Encephalitis Virus wild strain infection suppresses dendritic cells maturation and function, and causes the expansion of regulatory T cells

Figure 4

Effects of P3 infection on DCs activation of naïve T cells by MLR. Mock-treated, P3-infected or UV-P3-stimulated DCs as well as differently treated spDCs were added in grade dose to 1 × 105 allogeneic T cells at the indicated stimulator-responder ratios in triplicate, with (B) or without (A) LPS treatment for 20 h before the addition of 50 μl of CellTiter 96® AQueous One Solution Cell Proliferation Assay. The bmDCs, spDCs as well as T cells were served as spontaneous NADH/NADPH releases controls respectively. The presentation activities of differently treated bmDCs were measured as 100% (OD490DC+T exp.-OD490DC spont.-OD490T spont.)/(OD490T spont.). Results were expressed as the means ± SD of triplicates. *, P < 0.05.

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Virology Journal

ISSN: 1743-422X