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Figure 1 | Virology Journal

Figure 1

From: Japanese Encephalitis Virus wild strain infection suppresses dendritic cells maturation and function, and causes the expansion of regulatory T cells

Figure 1

P3 infects DCs in vitro and in vivo. (A) The in vitro infected bmDCs and the spDCs from P3-challenged mice were harvested and analyzed with RT-PCR. Bands shown are 467-bp PCR products specific for JEV. (B) The bmDCs and spDCs were analyzed for E protein (JEV envelope protein) by separation of the proteins on a 10% SDS-PAGE gel followed by electrotransfer to NC membranes and incubation with monoclonal antibodies against E protein. (C) The bmDCs were harvested after 3 days infection and the spDCs were isolated from mice which had been challenged for 5 days. 1 × 105 bmDCs or spDCs were doubly stained with FITC-anti-E and PE-anti-CD11c and analyzed by FACS respectively. (D) The infected bmDCs and the spDCs from challenged mice were collected 3 times at day 1, 3 and 5, and a real-time PCR was performed to quantitatively detect RNA copies of JEV. Each point represents the mean ± SD determinants in triplicate.

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Virology Journal

ISSN: 1743-422X