Table 1 Primers and probes developed and used in this study
Primers/Probes | Sequence 5'-3' | Label | Targeted ORF |
|---|---|---|---|
F-Sl-25S | CGCCCGGTCGTACTCATAA | none | |
R-Sl-25S | TCCATCGACCAGAGGCTGTT | none | NA |
P-Sl-25S | CGCATCAGGTCTCCA | VIC | |
PYMV-A-138-F | GCCTCTTGGCCCACTCTCTT | none | |
PYMV-A-201-R | GCCATTGAACGCCATGGA | none | CP |
PYMV-A-160-PMGB | ACTCAAAATGCCTAAGCG | FAM | |
PYMV-B-1356-F | TGCAGACTCTCCCGGATCTAG | none | |
PYMV-B-1415-R | CATCCGTATCGAGATCTGCAAA | none | MP |
PYMV-B-1378-PMGB | ACGCTTGCTCCCAGC | FAM | |
TYLCV-Mld-2186-F | CCTCTGACTTACTGCCTGAGTTAAGA | none | |
TYLCV-Mld-2246-R | GGTCAGCAGTCAGCCAATGA | none | C4 |
TYLCV-Mld-2213-PMGB | CTGCGGCGTAAGC | FAM | |
TYLCV-IL-2180-F | TGAGGGCCTCGGATTTATTG | none | |
TYLCV-IL-2241-R | CAATCTGCCAACGACGCATA | none | C4 |
TYLCV-IL-2201-PMGB | CTGAATTGAGTGCTTCGG | FAM | |
ToLCKMV-303-F | AGCGACCCGCCGATATAAT | none | |
ToLCKMV-361-R | TTCAGTCTCCGACGCACCTT | none | CP |
ToLCKMV-323-PMGB | ATTTCCACGCCCGCCT | FAM |