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Table 3 Primer combinations and annealing temperatures used to detect geminiviruses in Jatropha curcas and Manihot esculenta in PCR and RCA

From: Biotechnological approaches to determine the impact of viruses in the energy crop plant Jatropha curcas

Forward

primer

Reverse

primer

Length

Annealing

temperature

Part of genome amplified by PCR and RCA

JC1F

JC5R

1085 bp

60°C

PCR : amplifies part of AC1 and entire AC4

JC3F

JC5R

971 bp

63°C

PCR : amplifies part of AC1 and entire AC4

JC3F

JC4R

2800 bp

64°C

RCA : amplifies the entire DNA A

JC3F

JC2R

2800 bp

64°C

RCA : amplifies the entire DNA A

JC6F

JC4R

410 bp

55°C

PCR : amplifies part of AC2 and AC3

JC6F

JC2R

380 bp

55°C

PCR : amplifies part of AC1, AC2 and AC3

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Virology Journal

ISSN: 1743-422X