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Table 3 Primer combinations and annealing temperatures used to detect geminiviruses in Jatropha curcas and Manihot esculenta in PCR and RCA

From: Biotechnological approaches to determine the impact of viruses in the energy crop plant Jatropha curcas

Forward
primer
Reverse
primer
Length Annealing
temperature
Part of genome amplified by PCR and RCA
JC1F JC5R 1085 bp 60°C PCR : amplifies part of AC1 and entire AC4
JC3F JC5R 971 bp 63°C PCR : amplifies part of AC1 and entire AC4
JC3F JC4R 2800 bp 64°C RCA : amplifies the entire DNA A
JC3F JC2R 2800 bp 64°C RCA : amplifies the entire DNA A
JC6F JC4R 410 bp 55°C PCR : amplifies part of AC2 and AC3
JC6F JC2R 380 bp 55°C PCR : amplifies part of AC1, AC2 and AC3