Figure 8
From: The matrix (M) protein of newcastle disease virus binds to human bax through its BH3 domain
AF2240-M interacts with Bax. HeLa cells were co-transfected with pGFP-Bad, pGFP-Bax, pGFP-Bcl-XL or pEGFP.N2 and pFLAG-M, followed by lysis with 2% CHAPS buffer. Cell lysates were co-IPed with anti-FLAG M2-coated beads and Western blotted with either anti-FLAG M2 itself or anti-GFP. FLAG-BAP was used as control to evaluate successful IP. Total cell lysates were also directly Western blotted with either of the two antibodies. The double band at 38 kDa is non-specific. The amounts of total cell lysates loaded were verified by the actin.