Figure 10
From: The matrix (M) protein of newcastle disease virus binds to human bax through its BH3 domain
AF2240-M-ΔBH3, unlike AF2240-M, does not interact with Bax. HeLa cells were co-transfected with pFLAG-M or pFLAG-M-ΔBH3 and pGFP-Bax, followed by lysis with 2% CHAPS buffer. Cell lysates were co-IPed with anti-FLAG M2-coated beads and then Western blotted with either anti-FLAG M2 itself or anti-GFP. FLAG-BAP was used as control to evaluate successful IP. Total cell lysates were also directly Western blotted with either of the two antibodies.