Figure 3

Influenza virus accumulates in LAMP3 positive vesicles. (A) Localization of LAMP3 and early endosome marker EEA1. Hela cells were transfected with a LAMP3-GFP plasmid. Eighteen hours later, cells were stained with EEA1 antibody and analyzed by confocal microscopy. (B) LAMP3 translocated to plasma membrane during influenza virus infection. Hela cells transfected with LAMP3-GFP were infected with A/PR/8/34 virus at a MOI of 10 for 1 h. Cells were then fixed and analyzed by confocal microscopy. (C) LAMP3 colocolized with influenza A virus. Hela cells were transfected with LAMP3-GFP for 18 h and then infected with influenza A virus at 10 MOI. After 1 h, cells were stained with the anti-NP antibody and were subjected to confocal microscopy analysis.