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Figure 1 | Virology Journal

Figure 1

From: Lysosome-associated membrane glycoprotein 3 is involved in influenza A virus replication in human lung epithelial (A549) cells

Figure 1

LAMP3 is specifically induced upon influenza A virus infection. (A) Microarray results for LAMP1, LAMP2, and LAMP3 expression at different time points post influenza virus infection. A549 cells were infected with A/PR/8/34 virus at a MOI of 0.5. At indicated time points post-infection (p.i.), total cellular RNA was extracted and subjected to microarray analysis. (B) RT-PCR analysis for LAMP1, LAMP2, and LAMP3 expression. A549 cells were infected with A/PR/8/34 virus. After 12 h, 18, or 24 h, total cellular RNA was extracted and subjected to RT-PCR with specific primers. GAPDH gene was used as an internal control. (C) Western blot analysis for LAMP3 expression. A549 or Vero cells were treated as indicated in (B) and were lysed with RIPA buffer. Cell lysates were subjected to Western blot analysis with antibodies against LAMP3. β-actin was used as a loading control.

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