Skip to main content
Figure 2 | Virology Journal

Figure 2

From: Non-structural protein 1 of avian influenza A viruses differentially inhibit NF-κB promoter activation

Figure 2

Expression patterns of allele A and allele B NS1 proteins in subcellular compartments in A549 and MiLu cells. (A) A549 cells were transfected with vector encoding NS1 from H6N8-A, H6N8-B, H4N6-A, H4N6-B or empty pcDNA3.1+ vector (mock treated). 24 hours post-transfection cells were lysed and subjected to Western blotting and probed with anti-NS1 and β-actin antibodies. (B) A549 and MiLu cells were transfected with vector encoding NS1 from H6N8-A, H6N8-B, H4N6-A, H4N6-B. 18 hours post transfection, cells were fixed and processed for in situ PLA, as recommended.

Back to article page

Virology Journal

ISSN: 1743-422X