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Figure 1 | Virology Journal

Figure 1

From: Non-structural protein 1 of avian influenza A viruses differentially inhibit NF-κB promoter activation

Figure 1

Allele A and B NS1 proteins of avian influenza A viruses differentially block the dsRNA-induced expression of NF-κB promoter in A549 and MiLu cells. (A) A549 cells were transiently co-transfected with NF-κB luciferase reporter plasmid, and vector encoding NS1 from H6N8-A, H6N8-B, H4N6-A, H4N6-B or empty pcDNA3.1+ vector (mock treated) or left un-transfected (cells). 24 hours post transfection, cells were stimulated with dsRNA (+) or left un-stimulated (-). 24 hours post induction, cell extracts were prepared and luciferase activity was measured using the ONE-Glo™ Luciferase Assay System (Promega) following the manufacturer's Instructions. The values were normalized and pNF-κB cont. was set to 100%. (B) MiLu cells were transfected as described for A549 cells. All the conditions were maintained for MiLu cells as that of (A). Error bars indicate standard deviations. The data shown are representative for three experiments with transfections performed in duplicate. * indicates a significant difference as determined by the student's t-test, with p-values of < 0.05.

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