Multiple linear B-cell epitopes of classical swine fever virus glycoprotein E2 expressed in E.coli as multiple epitope vaccine induces a protective immune response

Table 3 Pigs immunized with rE2-a, rE2-b and rE2-ba elicited serum neutralizing antibodies early after viral challenge.

Groups	Pigs	Inoculum	Neutralizing antibody titer
			day p.i.					day p.c.
			0^a	7	14^b	21	28	35^c	42(7)	49(14)	56(21)	63(28)
A	5	rE2-a	< 2	< 2	4	16	32	32	64	64	64	64
B	5	rE2-b	< 2	< 2	8	32	64	64	128	128	128	128
C	5	rE2-ba	< 2	< 2	8	32	64	64	256	256	256	256
D	5	HCLV	< 2	< 2	8	16	32	64	128	128	128	128
E	5	PBS	< 2	< 2	< 2	< 2	< 2	< 2	< 2	< 2	< 2	0^d

Antibody titers were determined using a NPLA assay, as described in the study. Samples were collected every 7 days. a, first innoculation; b, second innoculation; c, CSFV strain Shimen challenge timepoint. d, all of the pigs in the group E had died after 28 dpc.

ISSN: 1743-422X