Skip to main content

Table 1 Effect of mutations at E1-226 and (E1-66/E1-70) on CHIKV infectivity for Ae. albopictus midgut cells

From: Chikungunya virus adaptation to Aedes albopictus mosquitoes does not correlate with acquisition of cholesterol dependence or decreased pH threshold for fusion reaction

Backbone

E1 226

Exp

N m

OID50

FL95

p-value

LR-GFP-226A

A

Com

194

5.45

NG

-

LR-GFP-226V

V

Com

261

3.52

NG

< 0.01

LR-GFP-226S

S

1

70

5.94

5.68-6.24

> 0.1

  

2

131

5.68

5.45-5.92

> 0.1

LR-GFP-226T

T

1

72

5.35

5.09-5.61

> 0.1

LR-GFP-226G

G

1

107

5.93

5.67-6.19

> 0.1

LR-GFP-226I

I

1

98

3.54

1.94-4.02

< 0.01

LR-GFP-226P

P

1

98

4.78

4.47-5.10

< 0.05

  

2

137

4.86

4.43-5.11

< 0.05

LR-GFP-226F

F

1

112

5.62

5.32-5.99

> 0.1

LR-GFP-226M

M

1

117

3.30

2.49-3.68

< 0.01

LR-GFP-226H

H

1

119

4.69

4.44-4.91

< 0.05

  

2

99

4.68

4.40-4.93

< 0.05

LR-GFP-226L

L

1

105

3.85

3.57-4.16

< 0.01

LR-GFP-Cl#1-10660A

V

1

80

3.41

3.32-3.50

< 0.01

  1. E1-226 - residues at position E1-226. Exp - experiment number. N m - number of mosquitoes used to estimate OID50 value. OID50 - oral infectious dose 50%, expressed as Log10TCID50/mL. FL95 - 95% fiducial limits for OID50 values expressed as Log10TCID50/mL. p-value - comparison of statistical significance of difference in OID50 values for CHIKV with indicated residues at E1-226 and OID50 value of LR-GFP-226A. Comb - combined summary of two independent experiments. NG - data not given.
Back to article page

Virology Journal

ISSN: 1743-422X