Figure 7

Effect of E1-A226V mutation on pH dependence of CHIKV induced cell-cell fusion. C6/36 cells infected with LR-GFP-226A or LR-GFP-226V viruses were incubated for 2 min with L-15 medium whose pH was previously adjusted to desired values. The reaction was abrogated by replacement of the fusion medium with 0.5 mL of standard L-15 medium. Percent of fusion was calculated as (1-c/n)x100%, where c is a number of eGFP expressing cells, n is number of nuclei (n≥70). Two independent experiments were performed for each virus (i and ii). Each individual experiment was performed in duplicates and results are expressed as an average of duplicates.