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Figure 6 | Virology Journal

Figure 6

From: Chikungunya virus adaptation to Aedes albopictus mosquitoes does not correlate with acquisition of cholesterol dependence or decreased pH threshold for fusion reaction

Figure 6

Effect of NH 4 Cl on competition between LR-ApaI-226V and LR-226A viruses for growth in C6/36 (A) and BHK-21 cells (B). Thirty minutes prior to infection cells were pre-incubated with L-15 media containing various amounts of NH4Cl. Then cells were infected with 1:1 mixture of LR-ApaI-226V and LR-226A viruses (107pfu/mL) and incubated for 48 h in L-15 media containing various amounts of NH4Cl. Cell culture supernatants were harvested and used for RNA extraction or titration on Vero cells. Virus RNA was processed as discussed previously [15].

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Virology Journal

ISSN: 1743-422X