Figure 6

Lung DC and IFN-α secretion following infection with RSV in immunized mice. BALB/c mice were immunized subcutaneously with AdF.RGD (10¹⁰ pu/mouse) or FIRSV (10⁵ pfu). A-C. Five wk later the mice were challenged intranasally with RSV (10⁶ pfu) for 6 days and the lung suspensions were analyzed for cDC (CD11b⁺,CD11c^high), pDC (CD11b^low, CD11c^high, B220⁺, PDCA-1⁺) and IFN-α secretion by flow cytometry and intracellular cytokine staining. A. cDC. B. pDC. C. IFN-α secretion by pDC. Data are presented as mean ± SEM of 4 mice/group from one of two independent experiments.** and * denote significance of p < 0.01 and p < 0.05, respectively.