Lung DC and IFN-α secretion following infection with RSV in immunized mice. BALB/c mice were immunized subcutaneously with AdF.RGD (1010 pu/mouse) or FIRSV (105 pfu). A-C. Five wk later the mice were challenged intranasally with RSV (106 pfu) for 6 days and the lung suspensions were analyzed for cDC (CD11b+,CD11chigh), pDC (CD11blow, CD11chigh, B220+, PDCA-1+) and IFN-α secretion by flow cytometry and intracellular cytokine staining. A. cDC. B. pDC. C. IFN-α secretion by pDC. Data are presented as mean ± SEM of 4 mice/group from one of two independent experiments.** and * denote significance of p < 0.01 and p < 0.05, respectively.