Frequency of RSV F protein-specific Th2-type CD4 and CD8 T cells and immunity against RSV in BALB/c mice following intramuscular immunization with AdF or AdF.RGD (1010pu/mouse). A, B. Ten days following immunization, spleen CD4 and CD8 T cells were co-cultured with splenic DC alone or with DC pulsed with recombinant smt3-RSV F protein or F peptide (DC-smt3-RSV F; DC-F85-93, respectively) and RSV F-specific cytokine responses were measured after 48 h by ELISPOT assay. A. RSV-specific IL-4 production in CD4 T cells; B. F epitope-specific production in CD8 T cells. C. RSV titer in the lungs of mice challenged by intranasal administration of RSV (106 cfu) 4 wk after immunization. Data represent mean ± SD of 5 animals/group from one of two independent experiments. ***, ** and * denote significance of p < 0.001, p < 0.01 and p < 0.05, respectively.