Table 1 Analytical reproducibility of one-step SYBR Green I RT-PCR assay in the inter- and intra- assay

The inter- assay

Ct values (mean ± S.D.)

31.63 ± 0.276

29.31 ± 0.482

25.56 ± 0.344

22.19 ± 0.286

18.55 ± 0.299

15.95 ± 0.300

24.10 ± 0.065

26.71 ± 0.570

28.87 ± 0.308

CV (%)

0.87

1.64

1.34

1.28

1.61

1.88

0.27

2.13

1.06

The intra- assay

Ct values (mean ± S.D.)

31.78 ± 0.274

29.25 ± 0.769

25.63 ± 0.327

22.30 ± 0.039

18.65 ± 0.142

16.08 ± 0.008

24.11 ± 0.046

26.71 ± 0.337

29.01 ± 0.266

CV (%)

0.86

2.63

1.27

0.17

0.76

0.05

0.19

1.26

0.91