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Figure 4 | Virology Journal

Figure 4

From: Development of one-step SYBR Green real-time RT-PCR for quantifying bovine viral diarrhea virus type-1 and its comparison with conventional RT-PCR

Figure 4

Comparison of one-step SYBR Green I RT-PCR with conventional RT-PCR for dectecting BVDV RNA of titrated viruses at 10-fold serial dilutions. (a) 2.5% agarose gel electrophoresis of the amplified products with a length of 84 bp obtained by one-step SYBR Green I RT-PCR. Marker: 50 bp DNA Marker, Lane 1: 100 TCID50, Lane 2: 10 TCID50, Lane 3: 1 TCID50, Lane 4: 10-1 TCID50, Lane 5: 10-2 TCID50, Lane 6: 10-3 TCID50, Lane 7: 10-4 TCID50, Lane 8: 10-5 TCID50. (b) 2.5% agarose gel electrophoresis of the amplified products with a length of 84 bp obtained by conventional RT-PCR. Marker: DNA Marker DL 500, lane 1: 100 TCID50, lane 2: 10 TCID50, lane 3: 1 TCID50, lane 4: 10-1 TCID50, lane 5: 10-2 TCID50, lane 6: 10-3 TCID50, lane 7: 10-4 TCID50, lane 8: 10-5 TCID50.

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Virology Journal

ISSN: 1743-422X