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Figure 4 | Virology Journal

Figure 4

From: Whole genome characterization of non-tissue culture adapted HRSV strains in severely infected children

Figure 4

Immunofluorescence examination of cells infected with the HRSV clinical strains. (A) Differential infection levels were observed with clinical isolates as compared to lab strain RSVA2. More HEp 2 cells were seen infected with RSVA2, when compared with clinical isolate RSV-8 and RSV-13 between 2 and 4 days post infection. (B). Clinical isolates (b) RSV-13, (c) RSV-8 and (d) RSV-6 also produced similar structures like (a) RSVA2. HEp2 cells were infected with RSVA2 and clincial isolates were stained with anti-RSV antibodies and visualized by immunofluorescence using secondary antibodies conjugated to FITC. Examination of the stained cells at a focal plane showing mainly the i) interior and ii) surface of infected cells are shown in each case. The presence of large cytoplasmic inclusion bodies highlighted by white arrow and presence of structures that resembled the VF are highlighted by star.

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