Figure 1

Schematic representation of HRSVA whole genome amplification of HRSV-1 by Reverse transcriptase (RT)-PCR. A. The viral genes with leader at 5' end and trailer at 3' end are schematically presented in the 15,222 bases HRSVA2 genome. The thin lines given below represents the approximate size of each of the fifteen amplified fragments (F) and the position of the respective fragments on the genome. The lines are to the approximate scale. B. All the PCR amplified fragments were run by electrophoresis on 1% agarose gel in TAE buffer and visualized by gel red. The fragments are numbered from F1-F15 and their positions on gel are indicated by arrows. Negative control (NC) is the PCR reaction with water in place of sample. The DNA ladder (L) has highest band position at 3 Kbp and lowest band at 100 bp. The size of fifteen fragments is as F 1 = 1215 bp, F2 = 1343 bp, F3 = 1417 bp, F4 = 1301 bp, F5 = 1373 bp, F6 = 1328 bp, F7 = 1324 bp, F8 = 1392 bp, F9 = 1435 bp, F10 = 1328 bp, F11 = 1403 bp, F12 = 1360 bp, F13 = 1200 bp, F14 = 1334 bp, F15 = 1168 bp.