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Figure 3 | Virology Journal

Figure 3

From: Development of an in situ assay for simultaneous detection of the genomic and replicative form of PCV2 using padlock probes and rolling circle amplification

Figure 3

Quantification of PCV2 infection in cells. PCV2 infection in PK-15A cells. a-f) Distribution of virus strand RCPs in nucleus and cytoplasm in individual cells at a) 24 h, b) 30 h, c) 48 h, d) 72 h, and e) 0 h after infection. f) negative control without ligase at 72 h after infection, g) fraction of cells with at least 15 RCPs in the nucleus, h) average number of RCPs in cells with more than 15 RCPs in the nucleus, j) average number of RCPs in nucleus are seen as white squares, in cytoplasm as crosses and totally per cell as black triangles from the padlock probe targeting the genomic strand and, k) average number of RCPs in nucleus are seen as white squares, in cytoplasm as crosses and totally per cell as black triangles from the padlock probe targeting the replicative strand.

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Virology Journal

ISSN: 1743-422X