Figure 1

Real-time -PCR quantification of HAdV 41 standard plasmid. A) Amplification plots of fluorescence intensities (ΔRn) versus the PCR cycle numbers are displayed for serial 10-fold dilutions of standard plasmids (1 × 10⁷ to 1 copy per reaction). B) Standard curve generated using a 10-fold dilution series (1 × 10⁷ to 1 copies per reaction) of HAdV 41 standard plasmid. Slope = -3.262, Y-intercept = 40.27, R² = 0.999.