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Figure 4 | Virology Journal

Figure 4

From: Herpes simplex virus type 1 UL14 tegument protein regulates intracellular compartmentalization of major tegument protein VP16

Figure 4

Localization of triple mutant UL14 proteins and VP5 in infected cells. The localization of wild-type and mutant UL14 proteins and capsid VP5 protein was observed in HEp-2 cells infected with UL14(3P)-VP16GFP or UL14(3P)R-VP16GFP viruses. Cells were infected at an MOI of 3, fixed at 11 h.p.i., permeabilized, and stained with either an anti-HSV-1 UL14 rabbit polyclonal antiserum (diluted 1:1000) [6], or an anti-HSV1+HSV2 ICP5 mouse monoclonal antibody (diluted 1:200).

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